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Annals of Medicine and Medical Education
Volume 1, Issue 2 (June 2014), pp. 43-52

DOI: 10.12973/ejms.2014.109p

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Research Article

Published online on Apr 06, 2014

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Assessment of Serum Indices Implementation on Roche Cobas 6000 Analyzer

Fatma Emel Koçak, Ayfer Meral, Havva Koçak


Background: Hemolyzed, lipemic and icteric samples which can be determined by naked eye can cause erroneous results and wrong 
diagnosis and treatments, so they are considered to be unsuitable and thus rejected. However, visual evaluation is subjective and is
not sufficiently reliable. The aim of this study was to evaluate efficiency and advantages of serum index program on the basis of test
parameters for detection of visually unrecognized interferences by analysing the samples in an automated system that include a serum
index program.

Methods: After examining through the naked eye, 717 serum samples which were decided to not be hemolyzed, lipemic and icteric were
picked out for the study. These samples were analyzed on Roche Cobas 6000 autoanalyzer for 23 different parameters which were known
to be most affected from interferences. At the same time, serum indices were also analyzed using Roche serum index application program.

Results: In 108 of 717 serum samples interference that previously could not be detected by naked eye was detected. 102 of these were
hemolysis, 4 were lipemia and 2 were icterus. Tests that were affected by hemolysis were creatine kinase isoenzyme MB(CK-MB), lactate dehydrogenase (LDH), aspartat aminotransferase (AST), total bilirubin, direct bilirubin, unsaturated iron binding capacity (UIBC), 

Conclusion: In detection of hemolytic, icteric and lipemic samples, visual detection is not reliable and automatized systems that gives serum
indices results based on the test parameters should take the place of visual detection as a cheap and fast way of accelerating the standard 
laboratory process and making it easier.

Keywords: Serum index program, sample diagnosis

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