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Annals of Medicine and Medical Education
Volume 2, Issue 2 (June 2015), pp. 30-47

DOI: 10.12973/ejms.2015.140p

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Research Article

Published online on Jun 01, 2015

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Effects of caffeic acid phenethyl ester in the prevention of stress ulcer in rats

Bircan Savran, Uğur Koltuksuz, Mahmut Şenyurt , Neşe Çallı Demirkan & Bünyamin Kaptanoğlu

Abstract

Background: Stress is one of the important factors responsible for the etiopathogenesis of many diseases. One of the diseases caused by stress is gastric ulcer. Gastric ulcer is called as stress ulcer when occurred because of stress. Purpose of this study was the investigation of caffeic acid phenethyl ester, an antioxidant and antiinflamatuar agent, effects on stress ulcer. Materials and Methods: Thirty Wistar Albino rats were used in this study. They were divided into three as control, stress and CAPE groups. “The cold-restraint method” was applied to induce stress ulcer to the stress and CAPE groups while controls did not. Stress and CAPE groups were given isotonic solution and 10 micromol/kg/day CAPE respectively once a week intraperitoneally three days before the stress application. Rats having stress were killed by cardiac puncture at the end of the experiment, and stomachs were removed and mean ulcer indices measured. Blood erythrocyte catalase (CAT) and nitric oxide (NO) as well as stomach tissue malondialdehyte (MDA) levels were analyzed. Results: At the end of study, it was seen that rats in CAPE group lost less weight than stress group. Severe hemorrhagic ulcers were observed in the stomach of all rats in stress group. Small ulcers like mucosal petechia were seen in CAPE group. It was determined that CAPE was 93.34% effective in preventing stress ulcer. Remarkable ulcerative lesions were observed microscopically in the stress group while there were minimal ulcerative lesions in CAPE group. CAT levels were found to be significantly higher in CAPE group than stress group (p=0.001) while lower than controls significantly (p=0.043). MDA levels were low in CAPE group compared to stress group (p=0.143) while high compared to controls (p=0.009). NO values of CAPE and stress groups were higher than those of controls (p=0.000). Conclusion: It was found that CAPE effectively prevented stress-dependent ulcer development in our study. The most important sign of this was the remarkably high inhibition percentage of CAPE. According to the findings obtained from this study CAPE prevented neutrouphil infiltration to gastric mucosa, and decreased mucosal damage considerably. With these results it was thought that CAPE probably prevented stress ulcer by blocking lipid peroxidation cascade triggered by free oxygen radicals.

Key Words: stress ulcer, caffeic acid phenethyl ester, cholestasis.


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