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Eurasian Journal of Anthropology
Volume 6, Issue 2 (December 2015), pp. 39-52

DOI: 10.12973/ejanth.2014.00044a

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Research Article

Published online on Jun 01, 2016

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Genetic characterization of Iranian subpopulations using 15 forensic autosomal STR loci

Mostafa Ghaderi-Zefrehei, Amin Mortazavi, Zohreh Baratieh, Alireza Sabouri, Reza Alaeddini, Somayeh Heidari, Elahe Foroughimanesh


The objective of present study was to compare genetic structure of seven Iranian provinces and to understand the genetic relationship among these seven provinces (Isfahan, Fars, Hormozgan, Kerman, Chaharmahal & Bakhtiari, Kohgiluyeh & Boyer Ahmad, and Yazd). For this purpose, a set of 15 STR’s loci were investigated. This panel of STRs is standard forensic science genetic testing tool that we added some importance to them by putting them in genetic variation analysis. The results based upon Fisher exact test for Hardy-Weinberg equilibrium (HWE) indicated that the allele and genotype distributions were in accordance with Hardy-Weinberg expectations except for D16S539. From genetic diversity point of view, D2S1338 was among the best STR loci and Isfahan has the highest value in aspect of these indices. Our results indicated that high amount of gene flow have been from Isfahan to Fars, which should be further investigated. Also, we indirectly shown that those forensic motivated genetic markers could be used as reliable tool for genetic differentiation when sounds data are available. We did not mean to estimate forensic science specific measures e.g. probability match and etc. for current study.

Keywords: STR’s loci, genetic relationship, genetic diversity, Iranian population

  1. Akbari MT, Papiha SS, Roberts DF, Farhud DD. (1986) Population genetics of the Persians and other peoples in Iran. Z Morphol Anthropol 76:197-217.
  2. Bahmanimehr A, Nikmanesh F, Yepiskoposyan L. (2014) Paternal genetic landscape of Zagros region of Iran and its role in the gene flow in the populations of the region. Iran J Epidemiol 10(3):43-53.
  3. Bi IZ, Maquet A, Baudoin J-P. (2003) Population genetic structure of wild Phaseolus lunatus (Fabaceae), with special reference to population sizes. Am J Bot 90(6):897-904.
  4. Excoffier L, Laval G, Schneider S. (2005) Arlequin (version 3.0): an integrated software package for population genetics data analysis. Evol Bioinform 1-47.
  5. Kalinowski ST. (2004) Counting alleles with rarefaction: private alleles and hierarchical sampling designs. Conserv Genet 5(4):539-543.
  6. Kummee P, Tangkijvanich P, Poovorawan Y, Hirankarn N. (2007) Association of HLA‐DRB1* 13 and TNF‐α gene polymorphisms with clearance of chronic hepatitis B infection and risk of hepatocellular carcinoma in Thai population. J Viral Hepatitis 14(12):841-848.
  7. Lahmi R, Vallian S. (2009) Genetic variation of informative short tandem repeat (STR) loci in an Iranian population. Iran J Biotechnol 7(3):137-141.
  8. Mojtabavi NM, Vallian BS, Hashemzadeh Chaleshtori M. (2013) Genetic variation of D7S2420 Marker in five ethnic groups of the Iranian Population: A highly informative marker for molecular diagnosis of ARNSHL. Genetics in the 3rd millennium 11(1):2960-2969.
  9. Moritz C, Dowling TE, Brown WM. (1987) Evolution of animal mitochondrial DNA: relevance for population biology and systematics. Annu Rev Ecol Syst 18:269-292.
  10. Motamedi S, Hashemzadeh-Chaleshtori M, Mojtabavi NM, Teimori H. (2015) Genetic variation of D9S1837 Marker located at TMC1 gene in Iranian population. Journal of Shahrekord University of Medical Sciences 16(6):75-83.
  11. Peakall R, Smouse PE. (2012) GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research—an update. Bioinformatics 28(19):2537-2539.
  12. Piry S, Alapetite A, Cornuet JM, Paetkau D, Baudouin L, Estoup A. (2004) GENECLASS2: a software for genetic assignment and first-generation migrant detection. J Hered 95(6):536-539.
  13. Raymond M, Rousset F. (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86(3):248-249.
  14. Senemar S, Doroudchi M, Pezeshki AM, Bazrgar M, Torab-Jahromi, Ghaderi A, (2009) Frequency of cystathionine β-synthase 844INS68 polymorphism in Southern Iran. Mol Biol Rep 36:353-356.
  15. Szpiech ZA, Jakobsson M, Rosenberg NA. (2008) ADZE: a rarefaction approach for counting alleles private to combinations of populations. Bioinformatics 24(21):2498-2504.
  16. Vallian S, Moeini H. (2006) Genotyping of five polymorphic STR loci in Iranian province of Isfahan. Journal of Sciences, Islamic Republic of Iran 17(2):113-117.
  17. Yeh FC, Yang RC, Boyle TB, Ye ZH, Mao JX. (1997) POPGENE, the user-friendly shareware for population genetic analysis. Canada: University of Alberta 10.