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Eurasian Journal of Analytical Chemistry
Volume 10, Issue 3 (December 2015), pp. 187-194

DOI: 10.12973/ejac.2015.114a

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Research Article

Published online on Dec 01, 2015

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Determination of Ethoxysulfuron Residues in Sugarcane Juice followed by HPLC-PDA Detection and Confirmation of Residues by LC-MS/MS

Tentu Nageswara Rao , T. Benarji Patrudu, M. V. Basaveswara Rao & Karri. Apparao

Abstract

A simple, sensitive and inexpensive method was developed using solid-phase extraction, together with high performance liquid chromatographic method with PDA detection for determination of ethoxysulfuron residues.  The evaluated parameters include the extracts by silica gel column using methanol, dichloromethane and acetone solvents. The method was validated using sugarcane juice samples spiked with ethoxysulfuron at different fortification levels (0.03 and 0.3 µg mL-1). Average recoveries (using each concentration six replicates) ranged 85-92%, with relative standard deviations less than 2%, calibration solutions concentration in the range 0.01-10.0 µg mL-1 and limit of detection (LOD) and limit of quantification (LOQ) were 0.01 µg mL-1 and 0.03 µg mL-1 respectively. Finally, the sugarcane juice residue samples were re analyzed by LC-MS/MS for confirmation of ethoxysulfuron.

Keywords: HPLC-PDA, LC-MS/MS, Ethoxysulfuron and Sugarcane juice        


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