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Eurasian Journal of Analytical Chemistry
Volume 11, Issue 2 (March 2016), pp. 55-61

DOI: 10.12973/ejac.2016.121a

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Research Article

Published online on Mar 01, 2016

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Approaches Development and Validation of RP-HPLC Method for Estimation of Glimepiride in Rat Plasma-Application to Pharmacokinetic Studies

Alok S. Tripathi Anil P. Dewani, Anil V. Chandewar & Papiya Mitra Mazumder

Abstract

A simple and sensitive method was developed for estimation of Glimepiride (GLIM) in rat Plasma by reverse phase high performance liquid chromatography (RP-HPLC). The drug samples were extracted by liquid-liquid extraction with 300 µL of acetonitrile and 5 mL of diethyl ether. Chromatographic separation was achieved on C18 column using methanol: water (85:15 v/v) as mobile phase at a flow rate of 1ml/min and UV detection at 230 nm. The retention time of GLIM was found to be 2.5 min. The developed method was validated for accuracy, precision, linearity and recovery. Linearity studies were found to be acceptable over the range of 100 – 6000 ng/mL. The method was successfully applied for the analysis of rat plasma sample for application in pharmacokinetic study, bioavailability.

Keywords: glimepiride, RP-HPLC, rat plasma


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