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Eurasian Journal of Analytical Chemistry
Volume 12, Issue 1 (January 2017), pp. 45-53

DOI: 10.12973/ejac.2017.00143a

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Research Article

Published online on Dec 04, 2016

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Metals Determination by Microwave Digestion ICP-OES of Some Dietary Supplements and Diet Products in Turkey

Hale Seçilmiş Canbay, Mahmut Doğantürk


Inductively coupled plasma optical emission spectrometry (ICP-OES) analysis was applied to metals concenrations in ten samples of some dietary supplements and diet products in Turkey. The As, Cr, Zn, Pb, Cd, Co, Ni, Hg, Mn, Fe, Al and Cu contents of dietary supplements and diet products were determined using microwave digestion. The digestion method with acid mixture (HNO3 + HCl) was used to break down the organic material of dietary supplements and diet products. The results of analysis showed that the mean level of metals were  Ni 3.34 ± 0.01 µg/g, Zn 41.24 ± 0.63 µg/g, Mn 23.27 ± 0.25  µg/g, Fe 221.10 ± 1.53 µg/g, Al 18.46 ± 0.14 µg/g and Cu 7.76 ± 0.15 µg/g for dietary supplements and diet products. The concentration of other metals and heavy metals was not found sufficiently. Based on the results obtained in the current work has sensitivity, and accuracy. Calibration curves showed a good linearity for all elements (R2>0.999). The quantitation limit ranged 2.5 to 5  µg/L in dietary supplements and diet products. Recoveries were in the range 92.12-102.08%. The results obtained form this study have shown good accuracy and reproducibility.

Keywords: metal, dietary supplements, diet products, ICP-OES

  1. Federal Food, Drug, and Cosmestic Act  of 1938. Public Law,  75-717, 52 Stat  1040.
  2. Dietary Supplement Health and Education Act of 1994. Public Law, 103-417, 108 Stat 4325.
  3. Raman, P., Patino, L. C., & Nair, M. G. (2004).  Evaluation of metal and microbial contamination in botanical supplements. J Agriculture Food Chemist, 52, 7822–7827. doi:10.1021/jf049150+
  4. Garcia-Rico, L., Leyva-Perez, J., & Jara-Marini, M. E. (2007). Content and daily intake of copper, zinc, lead, cadmium and mercury from dietary supplements in Mexico. Food Chem Toxicol, 45,1599–1605. doi:10.1016/j.fct.2007.02.027
  5. Report of the Commission on Dietary SupplementLabels. Washington, DC: Department of Healthand Human Services, Office of Disease Prevention and Health Promotion. (1997). .gov/dietsupp/final.pdf
  6. Dietarysupplements: FDA may have opportunities to expandits use of reported health problems to over se eproducts (GAO-13-244). Washington, DC: Government Accountability Office. (2013).
  7. Bailey, R. L., Gahche, J. J., Lentino, C.V .,  Dwyer, J. T.,  Engel, J. S., Thomas, P. R.,  Betz, J. M.,  Sempos, C. T.,  & Picciano, M. F. (2011).  Dietary supplement use in the United States, 2003-2006. J Nutr, 141, 261-266. doi:10.3945/​jn.110.133025
  8. Szok, A. F., Kurzwa, M., Cichosz, M., & Szlyk, E. (2015). Elemental Analysis of Medicinal Herbsand Dietary Supplements. Analytical Letters, 48, 2626-2638. doi:10.1080/00032719.2015.1041031
  9.  Mosby, C. V., Glanze, W. D., & Anderson, K. N. (1996). Mosby Medical Encyclopedia, The Signet: Revised Edition. St. Louis.
  10.  Jaben, S., Shah, M. T., Khan, S., & Hakat, M. Q. (2010). Determination of majorandtraceelements in ten important folk therapeuticplants of Haripurbasin, Pakistan. JMPR, 4, 559-566. doi:10.5897/JMPR10.015 
  11.  Seidal, K., Jorgensen, N., Elinder, C. G., Sjogren, B, & Vahter, M. (1993). Fatal cadmium-induced pneumonitis. Scand J Work Environ Health, 19, 429–431. doi:10.5271/sjweh.1450
  12.  Barbee, Jr J.Y., & Prince, T. S. (1999). Acute respiratory distress syndrome in a welder exposed to metal fumes. South Med J,  92, 510–512. doi:10.1097/00007611-199905000-00012
  13.  Järup, L. (2003). Hazards of heavy metal contamination. British Medical Bulletin, 68, 167–182. doi:10.1093/bmb/ldg032
  14. Jaishankar, M., Tseten, T., Anbalagan, N., & Mathew, B.B. (2014). Toxicity, mechanism and health effects of some heavy metals. Interdiscip Toxicol, 7, 60–72. doi:10.2478/intox-2014-0009
  15.  Adeloju, S. B., & Young, T. M. (1995). Anodic stripping potentiometric determination of antimony in environmental materials. Anal Chim Acta, 302, 225–32. doi:10.1016/0003-2670(94)00452-r
  16.  Łozak, A., K. Sołtyk, Ostapczuk, P., & Fijałek, Z. (2002). Determination of selected trace elements in herbs and their infusions. Sci Total Environ, 289, 33–40.doi:10.1016/s0048-9697(01)01015-4
  17. Adamo, P., S. Giordano, S. Vingiani, Cobianchi,  R. C., & Violante, P. (2003). Trace element accumulation by moss and lichen exposed in bags in the city of Naples (Italy). Environmental Pollution, 122, 91–103. doi:10.1016/s0269-7491(02)00277-4
  18. Tüzen, M. (2003). Determination of heavy metals in soil, mushroom and plant samples by atomic absorption spectrometry. Microchemical Journal, 74, 289–97. doi:10.1016/s0026-265x(03)00035-3
  19. Dolan, S. P.,  Nortrup, D. A.,  Bolger, P. M.,  & Capar, S. G. (2003).  Analysis of Dietary Supplements for Arsenic, cadmium, Mercury, and Lead Using Inductively Coupled Plasma Mass Spectrometry. J Agric Food Chem,  51, 1307-1312. doi:10.1021/jf026055x
  20. Basgel, S., & Erdemoğlu, S. B. (2006). Determination of mineral and trace elements in some medicinal herbs and their infusions consumed in Turkey. Sci Total Environ, 359, 82–9. doi:10.1016/j.scitotenv.2005.04.016
  21. Kumar, A., Nair, A. G. C.,  Reddy, A. V. R., & Garg, A. N. (2007). Analysis of essential elements in Pragya-peya–a herbal drink and its constituents by neutron activation. J Pharm Biomed Anal, 37, 631–36. doi:10.1016/j.jpba.2004.11.051
  22.  Kauffman, J. F., Westenberger, B. J., Robertson, J. D., Guthrie, J., Jacobs, A., & Cummins, S. K. (2007). Lead in pharmaceutical products and dietary supplements. Regul Toxicol Pharmacol 2007, 48, 128–134. doi:10.1016/j.yrtph.2007.03.001
  23. Aberoumand, A.,  Deokule, S. S. (2009). Determination of elements profile of some wild edible plants. Food Anal Methods, 2, 116–19. doi:10.1007/s12161-008-9038-z.
  24. The American Herbal Products Association. (2009). Heavy Metals: Analysis and Limits in Herbal Dietary Supplements.  1-41.
  25.  Fei, T., D. Li, Z. Fengqun, L. Junhua, Hua, T., & Xiangzhong, K. (2010). Determination of trace elements in Chinese medicinal plants by instrumental neutron activation analysis. JRNC, 284, 507–511. doi:10.1007/s10967-010-0503-y
  26.  Gjorgieva, D., T. Kadifkova-Panovska, Bačeva, K., & Stafilov, T. (2010). Content of toxic and essential metals in medicinal herbs growing in polluted and unpolluted areas of Macedonia. Arh Hig Rada Toksikol, 61, 297–303. doi:10.2478/10004-1254-61-2010-2022
  27. Jabeen, S., M. T. Shah, S. Khan, & Hakat, M. Q. (2010). Determination of major and trace elements in ten important folk therapeutic plants of Haripur basin, Pakistan. Journal of Medicinal Plants Research, 4, 559–566.
  28. Tumir, H., Bošnir, J., Dragun Z., Tomić, S., Puntarić, D., & Jurak, G. (2010).  Monitoring metal and metalloid content in dietary supplements on the Croatian market. Food Control, 21, 885–889. doi:10.1016/j.foodcont.2009.12.005.
  29.  Jurca, T., Maiani, E., Braun, M., & Vicas, L. (2011). Metals Determination by Microwave Digestion ICP-OES of Some Supplements. Rev Chim (Bucharest), 62, 139-140.
  30. Korfali, S. I.,  Hawi,  T., & Mroueh, M. (2013). Evaluation of heavy metals content in dietary supplements in Lebanon. Chem Cent J, 7, 1-13.  doi:10.1186/1752-153X-7-10
  31. Szymczycha-Madeja, A., Welna, M., & Zyrnicki, W. (2013). Multi-Element Analysis, Bioavailability and Fractionation of Herbal Tea Products. J Braz Chem Soc, 24, 777-787. doi:10.5935/0103-5053.20130102
  32. EPA 3051A, (1998). US Environmental Protection Agency Method 3051A. Microwave assisted acid digestion of sediments, sludges, soils, and oils.
  33. Saltan, F. Z., & Secilmis Canbay, H. (2015). Determination of Heavy Metals and Nutrient Elements in Some Plants Medicinal Used in Eskişehir.  SDU  Journal of Natural and Applied Science, 19, 83-90.
  34.  Marcos A., Fisher G., Ree G., & Hill S. J. (1998). Preliminary study using trace element concentrations and a chemometrics approach to determine the geological origin of tea. J Anal Atomic Spectr, 113, 521–525. doi:10.1039/A708658J
  35. Srividhya, B., Subramanian, R., & Raj, V. (2011). Determination of Lead, Manganese, Copper, Zinc, Cadminium, Nickel and Chromium in Tea Leaves.  International Journal of Pharmacy and Pharmaceutical Sciences, 3, 257-258.